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Trichloroisocyanuric Acid (TCCA) may be an ideal solution for melanin bleaching after the IHC staining of melanin-containing tissues

Our study revealed that either potassium permanganate or potassium dichromate bleaching can partially destroy tissue antigen and efficiently bleach DAB. Hence, neither potassium permanganate nor potassium dichromate is an ideal solution for bleaching melanin-containing tissues in IHC studies. In an attempt to find an agent that would bleach melanin without affecting DAB, we treated melanin-containing tissue sections with another bleaching agent, trichloroisocyanuric acid (TCCA). Our results showed that this agent was able to completely bleach melanin and partially destroy tissue antigen, with limited effects on DAB. Therefore, TCCA may be an ideal solution for melanin bleaching after the IHC staining of melanin-containing tissues.
Trichloroisocyanuric Acid (TCCA) (Aiershi Pai Disinfectant; Shanghai Likang Disinfectant Hi-Tech Co. Ltd), potassium permanganate, and potassium dichromate (AR; Xilong Shantou Chemical Industrial Company Limited) were used in bleaching methods. Vimentin (V9), Ki-67 (GM001), Melanosome (HMB45) (Ready-to-use, Dako), and UltraView Universal DAB Detection Kit (Roche 760-500) were used in IHC staining.
Trichloroisocyanuric Acid (TCCA) is an organic chlorine disinfection of drinking water preparation, high chlorine content, low odor, can remove color, storage stability, soluble in water and no sediment exist, less disinfection factors for a variety of water sources. We do Laboratory and field experiments many times: the concentration of 1.4 ~ 3.4mg / L; temperature 5 ~ 30 ℃; PH5 ~ 9; time 10 to 30 minutes; in addition to the four factors orthogonal test temperature 5 ℃; pH9 two combinations sterilization rate of only 99.94%, the sterilization rate can reach 99.99%. TCCA low toxicity, mutagenicity. TCCA and SM-92 No. coagulant combined can make water purification and disinfection better.