About Us

Trichloroisocyanuric Acid (TCCA) on the growth of cultured phytoplankton using laboratory culture techniques

Trichloroisocyanuric Acid (TCCA) is a strong oxidant, with an active chlorine concentration of >90%. The solubility of TCCA is 12 g/L in water at 25°C. It can be degraded in acidic or alkaline solutions. Furthermore, TCCA is an antimicrobial agent and decolorizer with broad spectrum, high efficiency, and low toxicity, and is thus widely used in food hygiene. The TCCA disinfection tablet is now inexpensive and commercially available.8 As a laboratory reagent, it is relatively safe and can be conveniently purchased and prepared. As a result of potassium permanganate or potassium dichromate bleaching can bleach DAB and TCCA bleaching does not, TCCA might be an ideal solution for melanin bleaching after the IHC staining of melanin-containing tissues. It is worth noting that TCCA should be freshly prepared before each experiment, and used within 2 hours of its preparation. In addition, sections should not be incubated with TCCA for over 30 minutes.
In our preliminary experiments, we found that incubation with 10% H2O2 for 30 minutes at 65°C did not completely remove melanin. When the incubation temperature was changed to room temperature, potassium permanganate bleaching or potassium dichromate bleaching was still ineffective in bleaching melanin. However, when samples were treated with 10 g/L Trichloroisocyanuric Acid (TCCA) for 4 hours at room temperature, DAB could be clearly removed.
Select the dominant species of phytoplankton in coastal tide diatom (Pseudo-nitzschia pungens), globosa (Phaeocystis globosa), donghaiense (Prorocentrum donghaiense), Alexandrium algae (Alexandrium tamarense) and Scrippsiella algae (Scrippsiella trochoidea), studied chemotherapeutic agents Trichloroisocyanuric Acid (TCCA) on the growth of cultured phytoplankton using laboratory culture techniques. The results showed that, TCCA to promote the growth of phytoplankton in the lower concentration range and at high concentrations significantly inhibited. TCCA Toxic Effects of phytoplankton species have differences, tolerance dinoflagellate significantly stronger than other types. TCCA effect at high concentrations, the growth of dinoflagellates appear delay phenomenon: the exponential growth phase lag significantly increased specific growth rate and chlorophyll a content. From the long-term impact, under eutrophic condition of phytoplankton on TCCA increased tolerance. Therefore, TCCA and other similar toxic organic compounds may inhibit the proliferation of diatoms and other algae by promoting dinoflagellates, and thus have a profound effect on the phytoplankton community structure.